DNA microsatellite variability of Ongole Cattle

Nama : Ningsih Satriani (G04496033)
Pembimbing : Achmad Farajallah Muladno
Tanggal Lulus : 1 Februari 2001
Judul Skripsi : Keragaman Genetik Sapi Peranakan Ongole (PO) Berdasarkan Uji DNA Mikrosatelit
    Genetic variability of Ongole Cattle based on DNA microsatellite Analysis
Abstrak :  
Baru-baru ini DNA mikrosatelit banyak digunakan sebagai marker untuk mempelajari variasi genetik. DNA mikrosatelit merupakan rangkaian molekul DNA pendek yang susunan basanya berulang dan terdapat melimpah dalam genom eukariot. Dalam penelitian ini, enam lokus mikrosatelit digunakan untuk menetapkan tingkat variabilitas genetik dalam populasi sapi PO. Nilai heterozigositas (h heterozigositas rataan (H dan variasi genetik populasi dihitung dan dibandingkan. Penghitungan perbedaan antar subpopulasi dan jarak genetik dihitung untuk mengetahui derajat diferensiasi dalam subpopulasi. Selanjutnya, indeks fiksasi juga dihitung untuk mengetahui struktur kawin populasi atau pola seleksinya dihubungkan dengan alel polimorfik. Hasil penelitian menunjukkan bahwa sapi PO mempunyai variasi genotipe dan heterozigositas yang tinggi. Heterozigositas rataan pada empat subpopulasi berkisar 0,505-0,606. DNA mikrosatelit populasi sapi PO bersifat polimorfik. Dalam populasi tersebut, tidak terjadi diferensiasi genetik karena subpopulasi hidup terpisah (GST= 37,1%) yang disebabkan oleh campur tangan manusia. Selain itu, struktur kawin populasi juga tidak acak (nilai indeks fiksasi tidak mendekati nol).
Abstract :  
Recently microsatellite DNA is often used as a marker for studying the genetic variance. DNA microsatellite is a tandemly repeated sequence abundantly in the present eukariotic genome. In the present study, six microsatellite loci were used to asses the level of genetic variability among populations of PO cattle. Heterozygosity value (h} average heterozygosity (H) and population genetic variation of four different subpopulation were calculated and compared. Estimates of differences between subpopulations and genetic distances were calculated to understand the degree of gene differentiation among subpopulation. In addition, the fixation indices were estimated to understand the breeding structure of population or the pattern of selection associated with polymorphic alleles.  The result showed that PO cattle population exhibited the high genotype variation and high heterozygosity. The average heterozygosity of four subpopulations were 0,505-0,606. DNA microsatellite of PO cattle is found polymorphic. Among populations, there was not found any genetic differentiation (GST= 37,1%) because they were lived separately that caused by human intervention. The breeding structure is found not random (The fixation indices value was not nearing the zero).

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