Resume Metode Ekstraksi mtDNA Vendi Eko Susilo G352110071

Resume Metode Ekstraksi mtDNA

oleh Vendi Eko Susilo  G352110071



No Nama Hewan Sample Metode Genome Target
1 Drosophila whole body?Adult drosophila alkaline lysis mtDNA
2 collected duringautopsy by Dr. William (human) Hair the silica/guanidine thiocyanate (GuSCN) total genome
3 yeast species cells 1) boiling, 2) glass bead disruption, and3) a commercially available kit (Gen Toru-Kun Kit/GenTLE) total genome
4 Hornbill Feathers use a single extractions step with chelex as a extraction medium total genome
5 Insect eggs QIAamp® columns (Vallencia, CA) haploid genome, mtDNA
6 cow fecal RBB+C ?
7 bear blood Qiam kit total genome
8 chicken, goat, cattle and pigs Fecal QIAamp DNA stool purification kit total genome, include gut bacterial consortium
9 Human Human fecal –   UltraClean Fecal DNA extraction kit-   ExtractMaster Fecal DNA extraction kit total genome, include gut bacterial consortium
10 human semen FTA  technology haploid genome, mtDNA



  1. Rapid Isolation Method of Animal Mitochondrial DNA by the Alkaline Lysis Procedure. Koichiro Tamura 1 and Tadashi Aotsuka 1′: Biochemical Genetics, Vol. 26, Nos. 11/12, 1988
  2. A Silica-Based Mitochondrial DNA Extraction Method Applied to Forensic Hair Shafts and Teeth. Lori E. Baker,1 M.A.; William F. McCormick,1 M.S., M.D.; and Karla J. Matteson,2 Ph.D. (2001)
  3. Comparison of Different Methods for Extraction of Mitochondrial DNA from Human Pathogenic Yeasts. Yohko Yamada*, Koichi Makimura1,2, Hossain Mirhendi1, Kumiko Ueda1, Yayoi Nishiyama1,2, Hideyo Yamaguchi1,2 and Masako Osumi; Jpn. J. Infect. Dis., 55, 122-125, 2002
  4. DNA extraction, amplification and direct sequensing from horbill feathers. Philip A Morina, Jeane Messier, and David S woodroff; J.Sci.Soc. Thailand, 20 (1994) 31-34
  5. Human and Insect Mitochondrial DNA Analysis from Maggots. Jeffrey D. Wells, Ph.D.; Francesco Introna, Jr., M.D.; Giancarlo Di Vella, Ph.D., M.D.; Carlo P. Campobasso, M.D.; Jack Hayes,  Ph.D.; and Felix A. H. Sperling, Ph.D ; JOURNAL OF FORENSIC SCIENCES (2001).
  6. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Zhongtang Yu and Mark Morrison; BioTechniques 36:808-812 (May 2004)
  7. Techniques for application of faecal DNA methods to field studies of Ursids. S. K. WASSER, C. S. HOUSTON, G. M. KOEHLER,* G. G. CADD and S. R. FAIN; Molecular Ecology 1997, 6, 1091–1097
  8. A rapid and low-cost DNA extraction method for isolating Escherichia coli DNA from animal stools. Barnard, T. G.1*, Robertson, C. A.1, Jagals, P.1 and Potgieter, N.2; African Journal of Biotechnology Vol. 10(8), pp. 1485-1490, 21 February, 2011
  9. Comparison of methods for the extraction of bacterial DNA from human faecal samples for analysis by real-time PCR. E.A Nelson1, 2, E.A Palombo1, and S.R Knowles2; (2010).
  10. 10.  Application of FTA technology to extraction of sperm DNA from mixed body fluids containing semen. Yoshihiko Fujita, Shin-ichi Kubo; Y. Fujita, S.-i. Kubo / Legal Medicine 8 (2006) 43–47






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